Question: A breaking buffer contains 10mM Tris, pH 8.0, 150mM Nacl. The elution buffer is breaking buffer that also…

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A breaking buffer contains 10mM Tris, pH 8.0, 150mM Nacl. The elution buffer is breaking buffer that also contains 300mM imidazole. Describe how the instructor made the 0.5 L elution buffer for students using a 1M stock of Tris (121.1 g/mole)(pH8.0), 5M Nacl (MW= 54.88g/mole) and 1Mimidazole (68.08g/mole).A breaking buffer contains 10mM Tris, pH 8.0, 150mM Nacl. The elution buffer is breaking buffer that also contains 300mM imidazole. Describe how the instructor made the 0.5 L elution buffer for students using a 1M stock of Tris (121.1 g/mole)(pH8.0), 5M Nacl (MW= 54.88g/mole) and 1Mimidazole (68.08g/mole).